CASP3 polyclonal antibody
产品名称： CASP3 polyclonal antibody
英文名称： CASP3 polyclonal antibody
产品编号： PAB2069
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of CASP3.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to internal region of human CASP3.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Ammonium sulfate precipitation
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Radiation enhances caspase 3 cleavage of Rad51 in BRCA2-defective cells.
Brown ET, Robinson-Benion C, Holt JT.Radiat Res. 2008 May;169(5):595-601.
- 2.
- Differential expression of mycobacterial antigen MPT64, apoptosis and inflammatory markers in multinucleated giant cells and epithelioid cells in granulomas caused by Mycobacterium tuberculosis.
Mustafa T, Wiker HG, Morkve O, Sviland L.Virchows Arch. 2008 Apr;452(4):449-56. Epub 2008 Feb 12.
- 3.
- Identification of caspase 3 motifs and critical aspartate residues in human phospholipase D1b and phospholipase D2a.
Wright MH, Farquhar MJ, Aletrari MO, Ladds G, Hodgkin MN.Biochem Biophys Res Commun. 2008 May 2;369(2):478-84. Epub 2008 Feb 25.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of CASP3 polyclonal antibody (Cat # PAB2069) in NCI-H460 cell line lysates (35 ug/lane).CASP3 (arrow) was detected using the purified Polyclonal antibody (1 : 60 dilution).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CASP3 polyclonal antibody (Cat # PAB2069) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Entrez GeneID:
- 836
- Protein Accession#:
- NP_004337;P42574
- Gene Name:
- CASP3
- Gene Alias:
- CPP32,CPP32B,SCA-1
- Gene Description:
- caspase 3, apoptosis-related cysteine peptidase
- Omim ID:
- 600636
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq
- Other Designations:
- OTTHUMP00000165054,PARP cleavage protease,SREBP cleavage activity 1,Yama,apopain,caspase 3,caspase 3, apoptosis-related cysteine protease,cysteine protease CPP32,procaspase3
- Interactome
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- Adenocarcinoma
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- Clubfoot
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- Diabetic Nephropathies
- Disease Progression
- Edema
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- Gastrointestinal Stromal Tumors
- Genetic Predisposition to Disease
- Head and Neck Neoplasms